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mortar and pestle fo plant dna in india

mortar and pestle fo plant dna in india

MB523- HiPurA® 96 Plant Genomic DNA Purification Kit

MB523 HiPurA® 96 Plant Genomic DNA Purification Kit Kit Contents Product Code ... Mumbai - 400 086, India 23, Vadhani Industrial Estate,LBS Marg, Mumbai - 400 086, India. Tel. : (022) 4017 9797 / 2500 1607 ... Prechill the mortar and pestle to –20°C before use. 6. Dissolve 2.6 mg of Additive-I per 400 l of Lysis Buffer (PL) and heat the ...

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Life Technologies India -- Life Tech India

Life Technologies (India) Pvt. Ltd. is a supplier of products services to life science research, industrial biotech pharma sector in India and neighbouring countries - in the fields of Immunology, Stem Cell Research, Human Cytogenetics, Phytodiagnostics, Tissue Culture, Bio-safety, Bio-stability, Bio-defense, Discovery Biology including GMO testing.

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Evaluation of different pulverisation methods for RNA ...

Introduction: Quality and integrity of RNA are critical for transcription studies in plant molecular biology. In squash fruit and other high water content crops, the grinding of tissue with mortar and pestle in liquid nitrogen fails to produce a homogeneous and fine powered sample desirable to ensure a good penetration of the extraction reagent.

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Mechanical Disruption Methods: Grinding

Cryogenic Grinding with Mortar Pestle: Grinding frozen samples with liquid nitrogen using a mortar and pestle is a widely used method. The mortar and pestle are cleaned and placed in a Styrofoam tub or cooler where liquid nitrogen is poured or dispensed onto the mortar and pestle.

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Plant DNA Extraction Protocol - .NET Framework

Plant materials are among the most difficult for high quality DNA extractions. The key is to properly prepare the tissues for extraction. In most cases this involves the use of liquid nitrogen flash freezing followed by grinding the frozen tissue with a mortar and pestle.

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Indian Journal of Science Research

TN, India.Vitex negundo(F ive-leaved chaste tree) were collected from village of Erode district of Tamilnadu. Curcuma longa (Tur meric) were purchased from local market of Erode, TN, India.The plant parts were identified according to various literatures. 2. EXTRACTION Ten grams of the plant materials were pounded manually with mortar and pestle and

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K.K

Classical and modern plant breeding techniques 110 genomic DNA. The general procedure to achieve complete digestion of cell walls while minimising DNA shearing involves dehydration of plant tissue in liquid nitrogen and, while still frozen, ground to a powder using a pestle and mortar.

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RNA Isolation Kit - Rna Extraction Kit Manufacturer from ...

The GenQuik™ Plant RNA Extraction Kit provides an efficient method for isolating total RNA from plant tissues. The tissue homogenizer or mortar and pestle can homogenize the fresh or frozen plant samples in the presence of lysis solution. The membrane spin filter will capture the RNA as contaminants are passing through the filter by ...

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MB507- HiPurA® Plant Genomic DNA Miniprep Purification Kit

Sample Preparation (Manual Disruption with mortar and pestle) For Leaf Sample: Finely cut the leaf material before grinding. Midrib should be removed before cutting the leaves as midrib is a major source of carbohydrate contamination. Weigh 100 mg of the finely cut plant material and grind properly using a mortar and pestle in liquid nitrogen to a

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Evaluation of DNA Extraction Methods for RAPD, SSR and ...

utilized for DNA isolation from all the accessions. 1. DNeasy Plant Mini Kit Protocol Grind 100 mg of leaf tissue to a fine powder in liquid nitrogen using a mortar and pestle. Transfer powdered material to pre warmed AP1 buffer (400 l) in an Eppendorf tube followed by other steps as per the manufacturer’s (Qiagen) instructions. ®

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Mechanical Disruption Methods: Grinding

Cryogenic Grinding with Mortar Pestle: Grinding frozen samples with liquid nitrogen using a mortar and pestle is a widely used method. The mortar and pestle are cleaned and placed in a Styrofoam tub or cooler where liquid nitrogen is poured or dispensed onto the mortar and pestle.

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Evaluation of economical and rapid method of plant DNA ...

cals were used for DNA extraction instead of costly chemicals. Fifth, the NaOH-Tris method requires only a small quantity of plant tissue (10 mg) and there is no sample waste whereas in modified CTAB method re-quires large quantities of plant tissue and ground in a mortar and pestle

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Successful tips of DNA extraction and PCR of plants for ...

Aug 18, 2018  These standards are electrophoresed with 300 ng-is of plant-extracted DNA. For example, the purity of extracted DNA is 33% if the band densities of 100-ng λ-DNA and plant extract are the same. Purities of plant DNA extract are usually less than 10% only with the popular treatments with PCI (later described), RNase, and ethanol precipitation.

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Scientific Protocols - A high throughput DNA extraction ...

Feb 07, 2013  Mortar and pestle; Procedure. Turn on a water bath and set temperature at 65°C. Grind plant material with mortar and pestle in the presence of extraction buffer [200 mM Tris-HCL (pH 8.0), 200 mM NaCl and 25 mM EDTA, and 1% PVP]. Transfer the homogenate to 1.5 ml micro-centrifuge tube and vortex for 30 seconds after adding of 10% SDS solution.

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GenElute™ Plant Genomic DNA Miniprep Kit Protocol (G2N10 ...

Several methods exist for the disruption of plant material. One of the most effective and commonly used methods is to grind the tissue in liquid nitrogen with a mortar and pestle. The GenElute Plant Genomic DNA Miniprep Kit was developed based on this efficient method of disruption.

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mCTAB: a rapid plant genomic DNA extraction

75 mg of plant tissue in chilled mortar and pestle with 500 µl of extraction buffer (Table 2), mCTAB: a rapid plant genomic DNA extraction 4 the homogenate was incubated at 60 0 C for 45 minutes ...

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Life Technologies India: - FBS, Tissue Culture Media ...

Genomic DNA Isolation Reagent : Cat. No.: 20-600-50 Store at: Room Temperature: Product Description: EZ-DNA is a non-organic and ready to use reagent for the isolation of genomic DNA from samples of human, animal, plant, yeast, bacterial and viral origin. EZ-DNA is an improved version of the Chomczynski method (1), which is based on disruption of cells in a guanidine-detergent lysing solution ...

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Plant DNA Kit Product Manual - Bioline

Plant DNA Kit PLANT DNA ISOLATION 14 bioline.co h C ompany DNA Kits ISOLATE II Bloo N it ISOLATE II Plant A it ISOLATE II Blood DNA Kit is designed for ef cient isolation of high purity genomic D whole blood and other body uids. FEATURES Fast 30 min isolation prot oc ol High-quality, inhibitor-free DNA High-purity DNA: typical A 260 /A 280

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Method for quality DNA isolation from different endangered ...

The ratio of the DNA isolated from seeds and stems was poor in quality, which was also reflected on the gel (Figure 1). After failure of quality DNA from some mate-rials of plant species, we have just modified isolation of DNA protocol, that is, we detached Cetyltrimethylammonium bromide (CTAB) and Mercaptoethanol components.

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An improved method of DNA isolation from polysaccharide ...

DNA from plants with high polysaccharides and ... to a fine powder in a mortar and pestle using liquid ... 1 U Taq DNA polymerase (Bangalore Genei, India) and 30 ng template DNA. The reactions were subjected to initial denaturation at 94°C for 4 min, followed by 40 amplification cycles, each consisting ...

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RNA Isolation Kit - Rna Extraction Kit Manufacturer from ...

The GenQuik™ Plant RNA Extraction Kit provides an efficient method for isolating total RNA from plant tissues. The tissue homogenizer or mortar and pestle can homogenize the fresh or frozen plant samples in the presence of lysis solution. The membrane spin filter will capture the RNA as contaminants are passing through the filter by ...

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Indian Journal of Science Research

TN, India.Vitex negundo(F ive-leaved chaste tree) were collected from village of Erode district of Tamilnadu. Curcuma longa (Tur meric) were purchased from local market of Erode, TN, India.The plant parts were identified according to various literatures. 2. EXTRACTION Ten grams of the plant materials were pounded manually with mortar and pestle and

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Molecular authentication of Cissampelos pareira L. var ...

grown at the Centre for Medicinal Plants Research, Arya Vaidya Sala, Kottakkal, Kerala, India, using a modified CTAB method (Doyle and Doyle 1987). The leaf samples (0.2 g) were ground in liquid nitrogen using mortar and pestle and re-suspended in 2 mL of DNA extraction buffer [2 % CTAB, 1 % PVP, 1.4 M NaCl, 20 mM EDTA (pH

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Genomic DNA isolation method from fresh wheat leaf

dried, cut into1 to 1 – 2 cm length and placed in sterile mortar and pastel. After rapping with aluminum foil, the mortar and pestle (with the sample) were maintained in -70°C freezer for 12 h. Then, deep frozen leaves were quickly gridded using mortar and pestle and transferred into sterile 2.0ml Eppendorf tube using sterile spatula.

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Genetic diversity in natural populations of arborea ...

dried leaf tissue. The tissue was ground in liquid nitrogen by using mortar and pestle to Table 1 Details of the 19 G. arborea populations used in this study Country of origin Population of origin Type Code on map Latitude Longitude No. of samples India Kasa NS 9 20 17 0N73 15 E63 Sholayar NS 10 10 220N76 370E27 Nowgong NS 3 27 000 N93 500E33

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DNA Extraction Protocol for Plants with High Levels of ...

Nov 14, 2012  Mangroves and salt marsh species are known to synthesize a wide spectrum of polysaccharides and polyphenols including flavonoids and other secondary metabolites which interfere with the extraction of pure genomic DNA. Although a plethora of plant DNA isolation protocols exist, extracting DNA from mangroves and salt marsh species is a challenging task. This study describes a

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Nuclear ribosomal DNA – ITS region based molecular marker ...

Oct 01, 2018  Roots of Gmelina arborea (Gambhari) is a medicinally important raw drug traded in India. However, Gmelina asiatica and Mallotus nudiflorus are also found in the raw drug markets as Gambhari. The current study aims to identify molecular markers based on the nuclear ribosomal DNA – ITS1 region to distinguish the authentic species from substitute/adulterants.

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Mortar and Pestle for RNA Extraction - Molecular Biology

Mortar and Pestle for RNA Extraction - (Jan/31/2012 ) Mortar and Pestle for RNA Extraction -. Our lab usually disrupts tissue with a Polytron, but since we planned to do extract RNA from pancreas, we have to use mortar and pestle set to grind the tissue in liquid nitrogen. Our lab already has a set of Coorstek ceremic mortar and pestle.

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A Guide to Choosing a Mortar and Pestle Epicurious

Mar 17, 2020  A mortar and pestle is about as lo-fi as it gets, a literally ancient implement comprised of a sturdy bowl (the mortar) and heavy club (the pestle). Pressing and twisting the pestle against the ...

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Extraction of Plant DNA by Microneedle Patch for Rapid ...

several steps including: 1) mechanical grinding of plant tissues (e.g., leaves) using mortar and pestle, 2) CTAB-based cell lysis, 3) organic phase DNA extraction, and 4) alcohol-assisted DNA precipitation and purification (Figure 1a). Alternatively, a NaOH-based quick plant DNA

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Grinding Onion To Extract Dna - greenrevolution

Class practical or demonstration You can extract DNA ... or knives to chop material and a mortar and pestle to grind material for ... but onions, and fish eggs ... Read more impacts of a waste plant

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ÄDELSTEN Pestle and mortar, marble black - IKEA

ÄDELSTEN Pestle and mortar. Made of hard marble; crushes spices efficiently. Delivery and assembly prices not included. Article number 202.016.20. Product details. Product details. MRP Rs.1,399 (incl. tax) Made of hard marble; crushes spices efficiently. You can use two different sides to crush spices in as the mortar is reversible.

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Robert Thangjam Mizoram University - Academia

The isolation of high quality DNA from plants containing high levels of polysaccharides and polyphenols has been a difficult problem. The use of liquid nitrogen, mortar and pestle, and young leaves does not always favour in handling large sample sizes for analysis. We demonstrate an easy and rapid extraction process by modifying many existing ones.

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